肼(N 2 H 4 )是化学工业中应用广泛的原料，但同时肼具有极高的毒性。因此，开发高效的检测方法对于监测环境中的肼和评估肼的生物毒性至关重要。本研究报道了一种近红外比例荧光探针 (DCPBCl 2 -Hz)，用于通过偶联氯取代的 D-π-A 荧光团 (DCPBCl 2 -Hz ) 来检测肼) 到识别基团乙酰基。由于氯取代的卤素效应，荧光团具有更高的荧光效率和更低的pKa值，适用于生理pH条件。肼可以与荧光探针的乙酰基发生特异性反应，释放出荧光团DCPBCl 2，使探针体系的荧光发射波长从490 nm显着偏移到660 nm。该荧光探针具有选择性好、灵敏度高、斯托克斯位移大、适用pH范围广等优点。装载探针的硅胶板可以实现方便地检测含量低至1 ppm（mg/m 3）的气态肼。随后，DCPBCl 2-Hz 成功应用于检测土壤中的肼。此外，该探针还可以穿透活细胞，使细胞内的肼可视化。可以预期，探针 DCPBCl 2 -Hz 将成为生物和环境应用中检测肼的有用工具。
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A near-infrared ratiometric fluorescent probe for hydrazine and its application for gaseous sensing and cell imaging
Hydrazine (N2H4) is a widely used raw material in the chemical industry, but at the same time hydrazine has extremely high toxicity. Therefore, the development of efficient detection methods is crucial for monitoring hydrazine in the environment and evaluating the biological toxicity of hydrazine. This study reports a near-infrared ratiometric fluorescent probe (DCPBCl2-Hz) for the detection of hydrazine by coupling a chlorine-substituted D-π-A fluorophore (DCPBCl2) to the recognition group acetyl. Due to the halogen effect of chlorine substitution, the fluorophore has an elevated fluorescence efficiency and a lowered pKa value and is suitable for physiological pH conditions. Hydrazine can specifically react with the acetyl group of the fluorescent probe to release the fluorophore DCPBCl2, so the fluorescence emission of the probe system significantly shifted from 490 nm to 660 nm. The fluorescent probe has many advantages, such as good selectivity, high sensitivity, large Stokes shift, and wide applicable pH range. The probe-loaded silica plates can realize convenient sensing gaseous hydrazine with content down to 1 ppm (mg/m3). Subsequently, DCPBCl2-Hz was successfully applied to detect hydrazine in soils. In addition, the probe can also penetrate living cells and allow the visualization of intracellular hydrazine. It can be anticipated that probe DCPBCl2-Hz will be a useful tool for sensing hydrazine in biological and environmental applications.